TEVET strategic partner, Agilent Technologies recently introduced the first expansion of their SureGuide pooled CRISPR libraries for functional genomics, offering pooled libraries for CRISPR activation and interference (CRISPR a/i). Typically, genetic screens using pooled libraries locate and identify genes involved in cellular response - such as in signaling pathways, or to discover the function of novel genes. With CRISPR a/i libraries, target transcription sites throughout the genome is possible. Also, every SureGuide CRISPR library is synthesized using Agilent's advanced SurePrint DNA synthesis platform - simultaneously printing billions of DNA bases with industry-leading sequence fidelity and representation. Overall, the new expansion enables scientists to turn up, or down, the expression of individual genes and screen the resulting phenotypes.
The completely flexible SureGuide CRISPR a/i libraries are offered in both Ready-to-Clone and Ready-to-Amplify formats. Ready-to-Clone libraries enable you to specify the sequence of each guide in the library or choose from validated predefined subsets. Ready-to-Amplify libraries allow you to fully design every aspect of the library including the tracer sequence. Therefore, you can use alternative delivery systems, guide structures, and cloning approaches while targeting any organism of choice.
The new libraries complement existing genome-wide libraries with promises of accelerating functional genomics research. Call a TEVET expert to get started today!
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